control dimethyl sulfoxide dmso group  (MedChemExpress)

Journal: Toxicology letters

Article Title: Loss of BRCA1 leads to an increased sensitivity to Bisphenol A

doi: 10.1016/j.toxlet.2010.09.008

Figure Lengend Snippet: Brca1f/f;MMTV-Cre or wild-type female mice were treated with BPA (A, C) or DMSO (B, D) as described in 2.5. Thick arrows indicate areas of abnormal dense mammary epithelial cell growth at the end of terminal ducts (TDs) in BPA-treated Brca1f/f;MMTV-Cre mice compared to ductal structure in DMSO-treated Brca1f/f;MMTV-Cre mice (thin arrows). Slightly increased alveolar buds (ABs) are present in BPA-treated Brca1f/f;MMTV-Cre mice compared to normal appearing Abs in DMSO-treated wild-type mice (thin arrows). Magnification, 40 X.

Article Snippet: Mice were weighed at 3 months of age and implanted with Alzet osmotic pumps (Alza Corp, Palo Alto, CA) designed to deliver either 50 % dimethyl sulfoxide (DMSO) (vehicle control, n=13/group) or 250 ng BPA/kg/body wt/day dissolved in DMSO (Sigma, St. Louis, MO) (n=13/group) for 4 weeks at a flow rate of 0.22 µl/h.

Techniques:

Journal: Toxicology letters

Article Title: Loss of BRCA1 leads to an increased sensitivity to Bisphenol A

doi: 10.1016/j.toxlet.2010.09.008

Figure Lengend Snippet: (A) Atypical ductal hyperplasia with formations of micro lumen was observed in BPA-treated Brca1f/f;MMTV-Cre mice. (B) DMSO-treated Brca1f/f;MMTV-Cre mice showed mild ductal hyperplasia. (C) BPA-treated wild-type mice showed few foci of mild ductal hyperplasia compared to (D) DMSO-treated wild-type mice where no structural changes of mammary gland were observed. Magnification, 40×; inset magnification, 100×.

Article Snippet: Mice were weighed at 3 months of age and implanted with Alzet osmotic pumps (Alza Corp, Palo Alto, CA) designed to deliver either 50 % dimethyl sulfoxide (DMSO) (vehicle control, n=13/group) or 250 ng BPA/kg/body wt/day dissolved in DMSO (Sigma, St. Louis, MO) (n=13/group) for 4 weeks at a flow rate of 0.22 µl/h.

Techniques:

Journal: Toxicology letters

Article Title: Loss of BRCA1 leads to an increased sensitivity to Bisphenol A

doi: 10.1016/j.toxlet.2010.09.008

Figure Lengend Snippet: (A) Increased PCNA staining was observed in mammary gland hyperplastic epithelial tissues of the BPA-treated Brca1f/f;MMTV-Cre mice. Mammary gland epithelial tissues of (B) DMSO-treated Brca1f/f;MMTV-Cre mice or (C) BPA-treated wild-type also showed frequent PCNA positives, comparing to (D) those of DMSO-treated wild-type mice. Magnification, 40×. (E) Differential cell proliferation index was found for each genotype and treatment of combination (DMSO or BPA).

Article Snippet: Mice were weighed at 3 months of age and implanted with Alzet osmotic pumps (Alza Corp, Palo Alto, CA) designed to deliver either 50 % dimethyl sulfoxide (DMSO) (vehicle control, n=13/group) or 250 ng BPA/kg/body wt/day dissolved in DMSO (Sigma, St. Louis, MO) (n=13/group) for 4 weeks at a flow rate of 0.22 µl/h.

Techniques: Staining